Functional Genomics

Through this core facility, MBICR researchers will have access to the following pooled libraries in retroviral or the third generation lentiviral vectors:

·????? Human full genome gRNA library

·????? Mouse full genome gRNA library

·????? Human full genome shRNA library

·????? Human fetal brain gene retroviral expression library

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The core facility will have the following reagents in 96-well and 384-well plates:

·????? Human genome-wide microRNA expression clones

·????? Human druggable gene expression clones

·????? Human apoptosis, autophagy and oncogene expression clones

·????? Oncogenic pathway reporter gene expression clones

·????? Human druggable gene esiRNA library

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万博体育 九州 365365体育会不会倒闭365bet体育在线手机版The facility is equipped with?two MDF-U74V -80C freezers and two MPR-1411-PC refrigerators from Panasonic, five PCR machines ( Two iCycle and one DNA Engine ALD1244 PCR machines from Biorad, One DNA Engine DYAD and one gradient cycler PTC-200 from MJ Research), various repeater and multichannel pipettes (Rainin EDP3 multichannel pipettes, Eppendorf Repeater Stream pipettes, Labsystems Finnpipette repeater pipettes and Thermo Scientific multichannel pipettes), two Multiskan Ascent microplate readers, one Nanodrop One, a Panasonic MLS-3781L autoclave machine, one Sorvall LYNX 4000 high-speed centrifuge from Thermo Scientific and two Stat Fax-2200 from AWARENESS Technology Inc.

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The functional genomics core facility in conjunction with the high-throughput screening core facility allows MBICR researchers and others in the southwest China area to conduct the large-scale functional analysis of genes that otherwise would not be practical.

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